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Catalog Number | orb1238532 |
---|---|
Category | Antibodies |
Description | DAPK3 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, ICC, IF, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | ZIP kinase antibody was raised against a peptide corresponding to amino acids near the center of human ZIP kinase.The immunogen is located within amino acids 270 - 320 of ZIPK. |
Concentration | 1 mg/ml |
Dilution range | ZIPK antibody can be used for detection of DNase II expression by Western blot at 1 μg/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Predicted: 26 kDa Observed: 27 kDa |
Target | DAPK3 |
UniProt ID | O43293 |
NCBI | BAA81746 |
Storage | ZIPK antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | ZIPK Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | ZIPK Antibody: ZIP, ZIPK, Death-associated protein Read more... |
Note | For research use only |
Application notes | ZIPK antibody can be used for detection of DNase II expression by Western blot at 1 μg/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of ZIP kinase in (A) HeLa and (B) Jurkat lysates with ZIP kinase antibody at 1 µg/mL.
Immunofluorescence of ZIPK in Jurkat cells with ZIPK antibody at 10 µg/mL.
Immunocytochemistry of ZIPK in Jurkat cells with ZIPK antibody at 10 µg/mL.
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IF, WB | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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