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Catalog Number | orb1239920 |
---|---|
Category | Antibodies |
Description | DAD1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, ICC, IF, WB |
Predicted Reactivity | Bovine, Gallus, Porcine, Rat |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | DAD1 antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human DAD1.The immunogen is located within the last 50 amino acids of DAD1. |
Concentration | 1 mg/mL |
Dilution range | DAD1 antibody can be used for detection of DAD1 by Western blot at 0.5 to 2 μg/mL. Despite its predicted molecular weight, DAD1 migrates at ~22 kDa in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | DAD1 |
UniProt ID | P61803 |
NCBI | AAH09798 |
Storage | DAD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | DAD1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | DAD1 Antibody: OST2, Dolichyl-diphosphooligosaccha Read more... |
Note | For research use only |
Application notes | DAD1 antibody can be used for detection of DAD1 by Western blot at 0.5 to 2 μg/mL. Despite its predicted molecular weight, DAD1 migrates at ~22 kDa in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of DAD1 in HepG2 cell lysate with DAD1 antibody at (A) 0.5, (B) 1, and (C) 2 µg/mL.
Immunocytochemistry of DAD1 in HepG2 cells with DAD1 antibody at 10 µg/mL.
Immunofluorescence of DAD1 in HepG2 cells with DAD1 antibody at 10 µg/mL.
ELISA, IF, IHC | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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