D2HGDH Antibody

• Catalog Number: orb1259096
• Category: Antibodies
• Description: D2HGDH Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 322-521 of human D2HGDH (NP_689996.4).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 56kDa
• Target: D2HGDH
• UniProt ID: Q8N465
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: D2HGD, D-2-hydroxyglutarate dehydrogenase, mitocho
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using D2HGDH antibody (orb1259096) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.