Cytokeratin Antibody, Multi 4/5/6/8/10/13/18

• Catalog Number: orb248639
• Category: Antibodies
• Description: This antibody recognizes multi cytokeratins: 4, 5, 6, 8, 10, 13, and 18. It is a broad-spectrum cytokeratin antibody which has been reported to be used to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of cytokeratin markers in cancer research and tumor diagnosis.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: C11
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: A keratin-enriched preparation from cultured human A431 was used as the immunogen for this Multi Cytokeratin antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-4ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Multi Cytokeratin antibody is available for research use only.
• Entrez: 4, 3853, 3856, 8, 10, 3851, 3858, 18, 3875, 5, 3860, 13, 3852, 6
• Storage: Store the Multi Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Multi Cytokeratin antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human colon carcinoma and Multi Cytokeratin antibody (clone C11).

IHC testing of FFPE rat oviduct and Multi Cytokeratin antibody (clone C11).

Immunofluorescent staining of permeabilized human HeLa cells with Multi Cytokeratin antibody (clone C11, green) and DAPI nuclear stain (blue).

Flow cytometry testing of permeabilized human HeLa cells with Multi Cytokeratin antibody (clone C11); Red=isotype control, Blue=Multi Cytokeratin antibody.

SDS-PAGE analysis of purified, BSA-free Multi Cytokeratin antibody (clone C11) as confirmation of integrity and purity.