Cytokeratin 7, CK7 Antibody

• Catalog Number: orb1294307
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Cytokeratin 7
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the center region
• Dilution range: Western Blot 1:1000–1:2000 Immunofluorescence 1:200–1:500 Immunohistochemistry (Paraffin) 1:200–1:500
• Conjugation: Unconjugated
• Target: Cytokeratin 7, CK7
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-Cytokeratin 7 antibody - at 1/2000 dilution. Lysates at 45 μg per lane. Nitrocellulose membrane was then blocked an hour before being incubated with orb1294307 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human Breast cancer tissue labeling Cytokeratin 7 with orb1294307 at 1/300.

Immunohistochemical analysis of paraffin embedded Human Colorectal cancer tissue labeling Cytokeratin 7 with orb1294307 at 1/300.

Immunohistochemical analysis of paraffin embedded Human Liver cancer tissue labeling Cytokeratin 7 with orb1294307 at 1/300.

Immunohistochemical analysis of paraffin embedded Human Lymph node carcinoma labeling Cytokeratin 7 with orb1294307 at 1/300.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294307 Anti-Cytokeratin 7 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294307 Anti-Cytokeratin 7 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Mouse tissue labeling Cytokeratin 7 with orb1294307 at 1/200.

Immunohistochemical analysis of paraffin embedded Mouse tissue labeling Cytokeratin 7 with orb1294307 at 1/200.

Immunohistochemical analysis of paraffin embedded Mouse tissue labeling Cytokeratin 7 with orb1294307 at 1/200.

Immunohistochemical analysis of paraffin embedded Mouse tissue labeling Cytokeratin 7 with orb1294307 at 1/200.

Immunohistochemical analysis of paraffin embedded Mouse tissue labeling Cytokeratin 7 with orb1294307 at 1/200.

Side by side with VANTANA IVD grade antibody.