Cytokeratin 6 antibody

• Catalog Number: orb2276480
• Category: Antibodies
• Description: Cytokeratin 6 antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: Biorbyt-606R
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant fragment of human KRT6 (exact sequence is proprietary)
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: P48668, P02538, P04259
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: CK6A, CK6B, CK6C, CK6D, CK6E, Keratin Type II Cyto
• Note: For research use only
• Application notes: Positive Control: Tonsil: A strong CK6 staining should be seen in all epithelial cells of the surface epithelium except the basal cell layer where staining should be absent or weak. Negative Control: Tonsil: CK6 staining should be absent in lymphocytes and all other mesenchymal cells. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Colorectal adenocarcinoma with strong KRT6 positivity in about 50% of tumor cells.

Squamous cell carcinoma of the uterine cervix with strong Cytokeratin 6 positivity.

Ectocervix - Cervical squamous epithelium with strong cytokeratin 6 immunostaining of all cell layers except basal cells.