Cytokeratin 5/6 Antibody

• Catalog Number: orb1825706
• Category: Antibodies
• Description: Cytokeratin 5 is a basic protein that is closely related to Cytokeratin 6. They share similar tissue distribution and are found in various proportions in many non-keratinizing stratified squamous epithelia eg. tongue mucosa, as well as in basal epithelia of trachea, basal cells of epidermis, hair follicles, sebaceous and sweat glands of skin, luminal cells of the mammary gland, basal cells of prostate, urothelium, vagina and endocervical mucosa. Cytokeratins 5 and 6 are also expressed in basal cell epitheliomas, squamous cell carcinomas of skin, tongue, epiglottis and of the rectal-anal region. Cytokeratin 5/6 mAb is a useful aid for classification of epithelioid mesotheliomas.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: KRT5.6/8275R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Human Cytokeratin 5- and Cytokeratin 6-homologous recombinant protein fragments were used as the immunogen for the Cytokeratin 5/6 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Hazard Information: This Cytokeratin 5/6 antibody is available for research use only.
• UniProt ID: P02538, P13647
• Storage: Aliquot the Cytokeratin 5/6 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human prostate carcinoma tissue with Cytokeratin 5/6 antibody (clone KRT5.6/8275R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC testing of FFPE human brain tissue with Cytokeratin 5/6 antibody (clone KRT5.6/8275R) at 2 ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.