Cytokeratin 4 Antibody / KRT4

• Catalog Number: orb639768
• Category: Antibodies
• Description: Cytokeratin 4 (KRT4) is an intermediate filament protein associated with Cytokeratin 13 (KRT13). It is expressed in suprabasal cells of non-keratinized stratified squamous epithelium of esophagus, cornea, anus, larynx, pharynx and tongue. Decreased expression of KRT4 is associated with head and neck squamous carcinoma. It is helpful in differentiation of squamous cell carcinoma of esophagus origin from that of thyroid origin.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: KRT4/2804
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A recombinant human partial protein (amino acids 181-292) was used as the immunogen for this Cytokeratin 4 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Cytokeratin 4 antibody is available for research use only.
• Storage: Store the Cytokeratin 4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of permeabilized human A549 cells with Cytokeratin 4 antibody (clone KRT4/2804, green) and Reddot nuclear stain (red).

IHC staining of FFPE human skin with Cytokeratin 4 antibody (clone KRT4/2804). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Flow cytometry testing of permeabilized human HeLa cells with Cytokeratin 4 antibody (clone KRT4/2804); Red=isotype control, Blue= Cytokeratin 4 antibody.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Cytokeratin 4 antibody (clone KRT4/2804). These results demonstrate the foremost specificity of the KRT4/2804 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.