Cytokeratin 20 antibody

• Catalog Number: orb1151515
• Category: Antibodies
• Description: Rabbit monoclonal antibody to Cytokeratin 20
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: KRT20/4879R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A portion of amino acids 196-323 was used as the immunogen for the recombinant Keratin 20 antibody.
• Dilution range: Immunohistochemistry (FFPE): 2-4ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant Keratin 20 antibody is available for research use only.
• UniProt ID: P35900
• Storage: Aliquot the recombinant Keratin 20 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Keratin 20 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human colon tissue with recombinant Keratin 20 antibody (clone KRT20/4879R) at 2 ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colon carcinoma tissue with recombinant Keratin 20 antibody (clone KRT20/4879R) at 2 ug/ml. Inset: PBS instead of primary Ab to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Keratin 20 antibody (clone KRT20/4879R) as confirmation of integrity and purity.