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Catalog Number | orb768910 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Cytokeratin 18 (phospho-Ser52) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | The antiserum was produced against synthesized peptide derived from human Keratin 18 around the phosphorylation site of Ser52. AA range:21-70 |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
MW | 48 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-KRT18 antibody, anti-CYK18 antibody, anti-PIG Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of 3T3 293T using p-Cytokeratin 18 (S52) antibody. Antibody was diluted at 1:500.
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Keratin 18 (Phospho-Ser52) Antibody.
Immunofluorescence analysis of HeLa cells, using Keratin 18 (Phospho-Ser52) Antibody. The picture on the right is blocked with the phospho peptide.
IHC-P, WB | |
Rat | |
Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
Rabbit | |
Recombinant | |
Biotin |
IF, IHC-P, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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