Cytokeratin 18 Antibody

• Catalog Number: orb749635
• Category: Antibodies
• Description: This mAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: KRT18/1190
• Tested applications: IF, IHC-P, WB
• Reactivity: Human, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant human KRT18 protein was used as the immunogen for the Keratin 18 antibody.
• Dilution range: Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Keratin 18 antibody is available for research use only.
• UniProt ID: P05783
• Storage: Store the Keratin 18 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Keratin 18 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of permeabilized human HeLa cells with Keratin 18 antibody (clone KRT18/1190, green) and Reddot nuclear stain (red).

IHC: Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Keratin 18 antibody (clone KRT18/1190).

IHC: Formalin-fixed, paraffin-embedded rat colon stained with Keratin 18 antibody (clone KRT18/1190).

Western blot testing of human 1) stomach and 2) intestine lysate using Keratin 18 antibody (clone KRT18/1190). Predicted molecular weight ~48 kDa.

Western blot testing of human HCT116 cell lysate using Keratin 18 antibody (clone KRT18/1190). Predicted molecular weight ~48 kDa.

Western blot testing of human A549 and HEP-2 cell lysate using Keratin 18 antibody (clone KRT18/1190). Predicted molecular weight ~48 kDa.