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Catalog Number | orb639780 |
---|---|
Category | Antibodies |
Description | This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. |
Species/Host | Mouse |
Clonality | Recombinant |
Clone Number | rKRT18/1190 |
Tested applications | IF, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Mouse IgG1, kappa |
Immunogen | Recombinant human KRT18 protein was used as the immunogen for the recombinant Cytokeratin 18 antibody. |
Dilution range | Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This recombinant Cytokeratin 18 antibody is available for research use only. |
Storage | Store the recombinant Cytokeratin 18 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human colon carcinoma with recombinant Cytokeratin 18 antibody (clone rKRT18/1190). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon carcinoma with recombinant Cytokeratin 18 antibody (clone rKRT18/1190). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human samples with recombinant Cytokeratin 18 antibody. Predicted molecular weight ~48 kDa.
FC, ICC, IF, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FACS, IF, IHC-P, WB | |
Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, ICC, IHC-Fr, WB | |
Canine, Gallus, Hamster, Human, Mouse, Porcine, Rabbit, Rat, Zebrafish | |
Mouse | |
Monoclonal | |
Unconjugated |
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