Cytokeratin 13 Antibody / KRT13

• Catalog Number: orb639774
• Category: Antibodies
• Description: Cytokeratin 13 (KRT13) is the major acidic keratin, which together with KRT4, its basic partner, is expressed in the suprabasal layers of non-cornified stratified epithelia including tongue mucosa, esophagus, anal canal epithelium, tracheal epithelium, uterine cervix, and urothelium. Defects in the KRT13 gene are a cause of white sponge nevus of cannon (WSN), a rare autosomal dominant disorder, which predominantly affects non-cornified stratified squamous epithelia and is characterized by the presence of soft, white and spongy plaques in the oral mucosa. KRT13 has been used as a marker for non-keratinized squamous epithelium. It is also expressed in various squamous metaplasia, but it is down regulated in squamous dysplasia and squamous carcinoma.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: KRT13/2659
• Tested applications: IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A recombinant full-length human KRT13 protein was used as the immunogen for this Cytokeratin 13 antibody.
• Dilution range: Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Cytokeratin 13 antibody is available for research use only.
• Storage: Store the Cytokeratin 13 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Flow cytometry testing of permeabilized human HeLa cells with Cytokeratin 13 antibody (clone KRT13/2213); Red=isotype control, Blue= Cytokeratin 13 antibody.

IHC staining of FFPE human prostate carcinoma with Cytokeratin 13 antibody (clone KRT13/2213). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil with Cytokeratin 13 antibody (clone KRT13/2213). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Cytokeratin 13 antibody (clone KRT13/2213) as confirmation of integrity and purity.