Cytokeratin 10/13 Antibody

• Catalog Number: orb749937
• Category: Antibodies
• Description: This antibody recognizes cytokeratin 10 (56.5kDa) and cytokeratin 13 (53kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on tissue sections this mAb serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: DE-K13
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: A cytoskeletal preparation extracted from human ectocervical epithelium was used as the immunogen for the Cytokeratin 10/13 antibody.
• Dilution range: Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Cytokeratin 10/13 antibody is available for research use only.
• UniProt ID: P13646, P13645
• Storage: Store the Cytokeratin 10/13 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Cytokeratin 10/13 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC analysis of formalin-fixed, paraffin-embedded human tonsil stained with Cytokeratin 10/13 antibody (clone DE-K13).

Western blot testing of A431 cell lysate and Cytokeratin 10/13 antibody (clone DE-K13).

SDS-PAGE Analysis of Purified, BSA-Free Cytokeratin 10/13 Antibody (clone DE-K13). Confirmation of Integrity and Purity of the Antibody.

Immunofluorescent staining of MeOH-fixed human MCF7 cells with Cytokeratin 10/13 antibody (clone DE-K13, green) and Reddot nuclear stain (red).