Cytokeratin 10/13 antibody

• Catalog Number: orb43753
• Category: Antibodies
• Description: Mouse Monoclonal to Cytokeratin 10/13.
• Clonality: Monoclonal
• Clone Number: DE-K13
• Tested applications: IHC-Fr, IHC-P, WB
• Reactivity: Feline, Human
• Isotype: Mouse IgG2a
• Immunogen: Cytoskeletal preparation extracted from human ectocervical epithelium.
• Concentration: 1 mg/ml
• Dilution range: Immunohistochemistry (paraffin sections): Standard ABC technique (DAB+), recommended dilution: 10 μg/ml (1:100), positive tissue: tonsil. Pretreatment: 0.1% pepsin in 0.1 M HCl for 30 min at room temperature or Target Retrieval Solution - High pH. The antibody DE-K13 reacts exclusively with Cytokeratin 13. Immunohistochemistry (frozen sections): Use at an assay dependent dilution. Western blotting: recommended dilution: 1-3 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: Cytokeratin 10/13
• RRID: AB_10992492
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-Cytokeratin 10+13 antibody
• Note: For research use only
• Application notes: Immunohistochemistry (paraffin sections): Standard ABC technique (DAB+), recommended dilution: 10 μg/ml (1:100), positive tissue: tonsil. Pretreatment: 0.1% pepsin in 0.1 M HCl for 30 min at room temperature or Target Retrieval Solution - High pH. The antibody DE-K13 reacts exclusively with Cytokeratin 13. Immunohistochemistry (frozen sections): Use at an assay dependent dilution. Western blotting: recommended dilution: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry staining of tonsil (paraffin-embedded sections) with anti-Cytokeratin 10+13, (DE-K13). The antibody DE-K13 stains only Cytokeratin 13 when used on formalin-fixed, paraffin-embedded tissue sections.