Cytokeratin 10 antibody

• Catalog Number: orb2276486
• Category: Antibodies
• Description: Cytokeratin 10 antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Biorbyt-610M
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Recombinant full-lenth human KRT10 protein.
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: P13645
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KR
• Note: For research use only
• Application notes: Positive Control: Skin: All squamous epithelial cells (except the basal cell layer) should show strong cytoplasmic staining, while the basal cell layer remains KRT10 negative or shows only minimal staining. Negative Control: Colon: All epithelial, inflammatory, stromal and muscle cells must not show any KRT10 staining. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Absence of KRT10 staining in non keratinizing squamous epithelium of the esophagus.

KRT10 is strongly positive in all suprabasal cell layers of the epidermis and in associated sebaceous glands and hair follicles. The basal cell layer remains strictly KRT10 negative.

Strong Cytokeratin 10 immunostaining in a squamous cell carcinoma of the vulva. The basal cell layers are largely unstained.