Cystatin C Antibody

• Catalog Number: orb1335109
• Category: Antibodies
• Description: Mouse monoclonal antibody to Cystatin C (CST3)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: OTI2C8
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Full length human recombinant protein of human CST3(NP_000090) produced in HEK293T cell.
• Dilution range: WB 1:2000, IHC 1:150
• Conjugation: Unconjugated
• MW: 13.3 kDa
• Target: Cystatin C
• UniProt ID: P01034
• NCBI: NM_000099
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts (35ug) from human plasma by using anti-CST3 monoclonal antibody (1:500).

Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-CST3 monoclonal antibody (1:500).

Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-CST3 monoclonal antibody (1:200).

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CST3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CST3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CST3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CST3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CST3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min)(1:150)

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CST3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CST3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CST3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CST3 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CST3.