CYP3A4 Antibody

• Catalog Number: orb1258123
• Category: Antibodies
• Description: CYP3A4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 244-503 of human CYP3A4 (NP_059488.2).
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 80kDa
• Target: CYP3A4
• UniProt ID: P08684
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: CYP3A4, cytochrome P450, family 3, subfamily A, po
• Note: For research use only
• Application notes: WB: 1:500 - 1:1000IHC: 1:20 - 1:50IF: 1:20 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using CYP3A4 antibody (orb1258123) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Immunohistochemistry of paraffin-embedded rat ovary using CYP3A4 antibody (orb1258123) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver using CYP3A4 antibody (orb1258123) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using CYP3A4 antibody (orb1258123) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using CYP3A4 antibody (orb1258123) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of mouse liver using CYP3A4 antibody (orb1258123) at dilution of 1:100. Blue: DAPI for nuclear staining.