CYP3A4 Antibody (Center)

• Catalog Number: orb1928893
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB15792
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 57343 Da
• Target: This CYP3A4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-255 amino acids from the Central region of human CYP3A4.
• UniProt ID: P08684
• NCBI: NP_059488.2, NP_001189784.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Cytochrome P450 3A4, 11413-,8-cineole 2-exo-monoox
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

CYP3A4 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

CYP3A4 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYP3A4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

All lanes: Anti-CYP3A4 Antibody (Center) at 1:1000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: A549 whole cell lysate. Lane 3: HT-29 whole cell lysate. Lane 4: OVCAR3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 57 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.