CYP21A2 Rabbit Polyclonal Antibody

• Catalog Number: orb13362
• Category: Antibodies
• Description: CYP21A2 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Canine, Equine, Human, Mouse, Porcine
• Reactivity: Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CYP21 (101-200/494aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 54 kDa
• Target: CYP21A2
• UniProt ID: P08686
• RRID: AB_10751404
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Cytochrome P450 21; cytochrome P450, family 21, su
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Jauregui, Estela J. et al. Leydig cell genes change their expression and association with polysomes in a stage-specific manner in the adult mouse testis Biol Reprod, 98, 722-738 (2018)

PubMed: 29408990

Tissue/Cell: rat testis tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CYP21 Polyclonal Antibody, Unconjugated (orb13362) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.