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CYP21A2 Antibody (Center)

Catalog Number: orb1928797

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb1928797
CategoryAntibodies
DescriptionPurified Rabbit Polyclonal Antibody (Pab)
Species/HostRabbit
ClonalityPolyclonal
Clone NumberRB16954
Tested applicationsFC, IF, IHC-P, WB
ReactivityHuman
IsotypeRabbit IgG
Antibody TypePrimary Antibody
Dilution rangeIF: 1:10~50, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
Form/AppearancePurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
ConjugationUnconjugated
MW56001 Da
TargetThis CYP21A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-246 amino acids from the Central region of human CYP21A2.
UniProt IDP08686
NCBINP_000491.4, NP_001122062.3
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Alternative namesSteroid 21-hydroxylase, 21-OHase, Cytochrome P-450
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NoteFor research use only
Expiration Date12 months from date of receipt.
CYP21A2 Antibody (Center)

Western blot analysis of CYP21A2 Antibody (Center) in MDA-MB435 cell line lysates (35 ug/lane). CYP21A2 (arrow) was detected using the purified Pab.

CYP21A2 Antibody (Center)

Confocal immunofluorescent analysis of CYP21A2 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

CYP21A2 Antibody (Center)

CYP21A2 Antibody (Center) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

CYP21A2 Antibody (Center)

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with CYP21A2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

  • CYP21A2 Antibody (Center) [orb1934393]

    WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl