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Catalog Number | orb1152433 |
---|---|
Category | Proteins |
Description | Cynomolgus IL-4, Fc Tag is expressed from human 293 cells (HEK293). It contains AA His 25 - Ser 153 (Accession # P79339-1). |
Reactivity | Monkey |
Tag | C-Fc |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 41.4 kDa |
Target | IL-4 |
UniProt ID | P79339 |
Source | Cynomolgus IL-4, Fc Tag (orb1152433) is expressed from human 293 cells (HEK293). It contains AA His 25 - Ser 153 (Accession # P79339-1). |
Expression System | HEK293 |
Biological Origin | Cynomolgus |
Biological Activity | Immobilized Cynomolgus / Rhesus macaque IL-4 R alpha, His Tag at 5 μg/mL (100 μL/well) can bind Cynomolgus IL-4, Fc Tag with a linear range of 0.1-4 ng/mL (QC tested). |
Expression Region | His 25 - Ser 153 |
Endotoxins | 1.0 EU per μg |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | 51 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 |
Alternative names | IL4,BCGF1,BSF1 Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 41.4 kDa. The protein migrates as 46-50 kDa and 53 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Immobilized Cynomolgus / Rhesus macaque IL-4 R alpha, His Tag (Cat. No. orb257988) at 5 μg/mL (100 μL/well) can bind Cynomolgus IL-4, Fc Tag (Cat. No. orb1152433) with a linear range of 0.1-4 ng/mL (QC tested).
Unconjugated | |
95% | |
25.6 kDa | |
Cynomolgus / Rhesus macaque IL-4 R alpha, His Tag (orb257988) is expressed from human 293 cells (HEK293). It contains AA Met 26 - Arg 232 (Accession # G7Q0S7). |
Unconjugated | |
95% | |
50.4 kDa | |
Cynomolgus / Rhesus macaque IL-4 R alpha, Fc Tag (orb257987) is expressed from human 293 cells (HEK293). It contains AA Met 26 - Arg 232 (Accession # G7Q0S7). |
98.00% | |
16.03 kDa (predicted). Due to glycosylation, the protein migrates to 23-28 kDa based on Tris-Bis PAGE result. |