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Catalog Number | orb1176549 |
---|---|
Category | Proteins |
Description | Cynomolgus IL-2, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Thr 154 (Accession # Q29615-1). |
Reactivity | Monkey |
Tag | N-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 17.4 kDa |
Target | IL-2 |
UniProt ID | Q29615 |
Source | Cynomolgus IL-2, His Tag (orb1176549) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Thr 154 (Accession # Q29615-1). |
Expression System | HEK293 |
Biological Origin | Cynomolgus |
Biological Activity | Cynomolgus IL-2 R beta, His Tagimmobilized on CM5 Chip can bind Cynomolgus IL-2, His Tag with an affinity constant of 377 nM as determined in a SPR assay (Biacore 8K) (Routinely tested). |
Expression Region | Ala 21 - Thr 154 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_001271628.1 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | IL2,TCGF,lymphokine,Interleukin 2 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 17.4 kDa. The protein migrates as 18 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Cynomolgus IL-2 R beta, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-2, His Tag (Cat. No. orb1176549) with an affinity constant of 377 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Unconjugated | |
90% | |
26.3 kDa | |
Cynomolgus IL-2 R alpha Protein, His Tag (orb1496308) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Glu 238 (Accession # H6WS54). |
98.00% | |
28.2 kDa (predicted). Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |
98.00% | |
28.2 kDa (predicted). Due to glycosylation, the protein migrates to 50-68 kDa based on Tris-Bis PAGE result. |
98.00% | |
25.6 kDa (predicted). Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |