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Catalog Number | orb570265 |
---|---|
Category | Proteins |
Description | Cynomolgus Complement C5, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Cys 1676 (Accession # XP_005580972.1). |
Reactivity | Monkey |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 73.9 kDa (β chain) & 114.9 kDa (α chain) |
Target | Complement C5 |
Source | Cynomolgus Complement C5, His Tag (orb570265) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Cys 1676 (Accession # XP_005580972.1). |
Expression System | HEK293 |
Biological Origin | Cynomolgus |
Biological Activity | Immobilized Cynomolgus Complement C5, His Tag at 2 μg/mL (100 μL/well) can bind Anti-C5a (Human IgG1) with a linear range of 0.4-6 ng/mL (QC tested). |
Expression Region | Gln 19 - Cys 1676 |
Endotoxins | 1.0 EU per μg |
NCBI | XP_005580972.1 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | Complement C5,C5,CPAMD4 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus.The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.9 kDa (β chain) and 114.9 kDa (α chain). The protein migrates as 65 kDa and 113 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Immobilized Cynomolgus Complement C5, His Tag (Cat. No. orb570265) at 2 μg/mL (100 μL/well) can bind Anti-C5a (Human IgG1) with a linear range of 0.4-6 ng/mL (QC tested).
98.00% | |
8.41 kDa (predicted); 12.42 kDa (reducing conditions) |
98.00% | |
188.1 kDa (predicted); 106.3 and 75.8 kDa (reducing conditions) |