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Catalog Number | orb1101181 |
---|---|
Category | Proteins |
Description | Cynomolgus Complement C3, His Tag is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Asn 1663 (Accession # G7PYU9-1). |
Reactivity | Monkey |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 71.3 kDa (β chain) and 113.0 kDa (α chain) |
Target | Complement C3 |
UniProt ID | G7PYU9 |
Source | Cynomolgus Complement C3, His Tag (orb1101181) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Asn 1663 (Accession # G7PYU9-1). |
Expression System | HEK293 |
Biological Origin | Cynomolgus |
Biological Activity | Immobilized Cynomolgus Complement C3, His Tag at 1 μg/mL (100 μL/well) can bind Human Anti-C3,Human IgG1 Human Kappa with a linear range of 0.2-8 ng/mL (QC tested). |
Expression Region | Thr 23 - Asn 1663 |
Endotoxins | 1.0 EU per μg |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | Complement C3,CPAMD1 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement component 3 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 71.3 kDa (β chain) and 113.0 kDa (α chain). The protein migrates as 64-68 kDa (β chain) and 100-120 kDa (α chain) and 160-180 kDa (intact Complement component 3) under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Immobilized Cynomolgus Complement C3, His Tag (Cat. No. orb1101181) at 1 μg/mL (100 μL/well) can bind Human Anti-C3, Human IgG1 Human Kappa with a linear range of 0.2-8 ng/mL (QC tested).
90.30% | |
186.40 kDa (predicted) |