Cyclophilin B Mouse Monoclonal Antibody (Loading Control)

• Catalog Number: orb526697
• Category: Antibodies
• Description: Cyclophilin B Mouse Monoclonal Antibody (Loading Control)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4C7
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Cyclophilin B
• Concentration: 1mg/ml
• Dilution range: WB=1:1000-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 20 kDa
• Target: PPIB
• UniProt ID: P23284
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Cphn 2; Cphn2; CyP 20b; CYP S1; CYP-S1; CYPB; MGC1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (human intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Cyclophilin B (Loading Control)) Monoclonal Antibody, Unconjugated (orb526697) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Cyclophilin B (Loading Control)) Monoclonal Antibody, Unconjugated (orb526697) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Cyclophilin B (Loading Control)) Monoclonal Antibody, Unconjugated (orb526697) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Cyclophilin B (Loading Control)) Monoclonal Antibody, Unconjugated (orb526697) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Cyclophilin B (Loading Control) Monoclonal Antibody, Unconjugated (orb526697) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Cyclophilin B (Loading Control) Monoclonal Antibody, Unconjugated (orb526697) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Fundus, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Cyclophilin B (Loading Control) Monoclonal Antibody, Unconjugated (orb526697) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Cyclophilin B (Loading Control) Monoclonal Antibody, Unconjugated (orb526697) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Cyclophilin B (Loading Control) Monoclonal Antibody, Unconjugated (orb526697) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Cyclophilin B (Loading Control) Monoclonal Antibody, Unconjugated (orb526697) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Cyclophilin B (Loading Control) Monoclonal Antibody, Unconjugated (orb526697) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Cyclophilin B (Loading Control) Monoclonal Antibody, Unconjugated (orb526697) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Thyroid Gland, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Cyclophilin B (Loading Control) Monoclonal Antibody, Unconjugated (orb526697) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Stomach, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Cyclophilin B (Loading Control) Monoclonal Antibody, Unconjugated (orb526697) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Thyroid Gland, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Cyclophilin B (Loading Control) Monoclonal Antibody, Unconjugated (orb526697) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug, Lane 2: A431 (Human) Cell Lysate at 30 ug, Lane 3: HepG2 (Human) Cell Lysate at 30 ug, Lane 4: Thyroid gland (Mouse) Lysate at 40 ug, Lane 5: Thyroid gland (Rat) Lysate at 40 ug, Lane 6: Placenta (Mouse) Lysate at 40 ug, Lane 7: Cerebrum (Mouse) Lysate at 40 ug, Lane 8: Cerebrum (Rat) Lysate at 40 ug, Lane 9: Liver (Mouse) Lysate at 40 ug, Lane 10: Liver (Rat) Lysate at 40 ug, Lane 11: Kidney (Mouse) Lysate at 40 ug, Lane 12: Kidney (Rat) Lysate at 40 ug, Lane 13: Huvec (Human) Cell Lysate at 30 ug, Lane 14: U937 (Human) Cell Lysate at 30 ug, Primary: Anti-Cyclophilin B (orb526697) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 20 kD, Observed band size: 20 kD.