Cyclooxygenase 2 Rabbit Polyclonal Antibody

• Catalog Number: orb182638
• Category: Antibodies
• Description: Cyclooxygenase 2 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat, Sheep
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Cyclooxygenase 2 (301-400/604aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: ICC/IF=1:50-200, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 65 kDa
• Target: Ptgs2
• UniProt ID: P35354
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: PTGS2; COX 2; COX2; COX-2; Cyclooxygenase; Cycloox
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Recombinant human COX2 protein, Primary: Anti-Cyclooxygenase 2 (orb182638) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65 kD, Observed band size: 40 kD.

Tissue/Cell: human myometrium tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Cyclooxygenase-2 Polyclonal Antibody, Unconjugated (orb182638) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: human prostate tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-COX-2 Polyclonal Antibody, Unconjugated (orb182638) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: mouse brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-COX-2 Polyclonal Antibody, Unconjugated (orb182638) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.