Cyclin Dependent Kinase Antibody Sampler Kit

• Catalog Number: orb342332
• Category: Assays and Kits
• Description: Cyclin Dependent Kinase Antibody Sampler Kit
• Tested applications: ELISA, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: User Optimized, IP: 1:100, WB: 1:500 - 1:5,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Specific Polyclonal Antibodies
• Conjugation: Unconjugated
• Storage: Store antibodies at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Anti-cdc2 (p34), Anti-cdk2, Anti-cdk4, Anti-cdk5,
• Note: For research use only
• Application notes: Each kit contains one unit of the following products: (100 µL) Anti-cdc2 (p34) (RABBIT) Antibody (100 µL) Anti-cdk2 (RABBIT) Antibody (100 µL) Anti-cdk4 (RABBIT) Antibody (100 µL) Anti-cdk5 (RABBIT) Antibody (100 µL) Anti-cdk9 (PITALRE) (RABBIT) Antibody (100 µg) Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated
• Expiration Date: Please enquire.

Biorbyt's anti-cdc2 Cyclin Dependent Kinase (orb750449) was used to detect human p34cdc2by western blot in untreated (Contol) and drug treated lysates of MCF-7 cells. Lane 1-4 represents 3.1?uM, 6.2?uM, 12.5?uM and 25.0?uM genistein treatment of cells before lysates were prepared. Detection occurs using a 1:1000 dilution. Although this antiserum detects non-specific bands at higher MW, a clear induction of signal is observed as the concentration of drug is increased.

Biorbyt's Anti-CDK2 antibody (orb750450) was diluted 1:500 to detect CDK2 in human skin tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Biorbyt's anti-CDK5 antibody (orb750453) was diluted 1:500 to detect CDK5 in human brain cortex tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Immunocytochemical staining of mouse tissue using anti-cdk9 (PITALRE) antiserum (orb750454). The staining shows the location of mcdk9/PITALRE protein in developing mouse tissue. Arrows indicate areas of high expression. Panel A: Peroxidase-DAB immunostaining of mcdk9/PITALRE protein in the developing mouse brain in the differentiated region of the medulla oblongata just below the fourth ventricle. Similar staining is shown in Panel B in the dorsal root ganglia. Panel C: Fluorescein immunofluorescence of mcdk9IPITALRE in skeletal muscle. Similar staining is shown in Panel D in cardiac muscle. Other detection systems should yield similar results. Sections from each specimen were cut at 5-7 µM, mounted on glass and dried overnight at 37°C. All sections then were deparaffinized in xylene, rehydrated through a graded alcohol series and washed in phosphate-buffered saline (PBS). PBS was used for all subsequent washes and for antiserum dilution. Tissue sections were quenched sequentially in 0.5% hydrogen peroxide and blocked with diluted 10% normal goat anti-rabbit serum. Slides were incubated at 20°C for 1 h with rabbit anti-cdk9 (1:500) dilution, washed, and then reacted with diluted goat anti-rabbit biotinylated antibody for 30 min. All the slides were then reacted with streptavidin-peroxidase conjugate for 30 min at 20°C. Diaminobenzidine was used as the final chromogen and hematoxylin was used as the nuclear counterstain. Negative controls for each tissue section were prepared by substituting the primary antiserum with pre-immune serum.

Rabbit anti-cdk2 (orb750450) was used at a 1:200 dilution to detect cdk-2 in asynchronous HeLa cell lysates (run in duplicate). Approximately 50 µg of lysate was separated on a 15% SDS-PAGE gel. Cdk-2 is indicated by an arrowhead as a 32-33 kDa band. Note that multiple isoforms as well as phosphorylated forms of cdk-2 may be detected by this antibody. Primary antibody was reacted at room temperature for 1 h. After subsequent washing, a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG (orb347654) preceded color development.