Cyclin D2 (phospho-Thr280) antibody

• Catalog Number: orb770787
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Cyclin D2 (phospho-Thr280)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Cyclin D2 around the phosphorylation site of Thr280. AA range:240-289
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• MW: 40
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-CCND2 antibody, anti-G1/S-specific cyclin-D2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from COLO205 cells treated with EGF 200ng/ml 30', using Cyclin D2 (Phospho-Thr280) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200.