Cyclin D1 Antibody / CCND1

• Catalog Number: orb750252
• Category: Antibodies
• Description: Cyclin D1 (CCND1), one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of Cyclin D1. This antibody is useful in identifying mantle cell lymphomas (CCND1+) from CLL/SLL and follicular lymphomas (CCND1-). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express CCND1.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SPM587
• Tested applications: FACS, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Mouse IgG2a, kappa
• Immunogen: Recombinant full length human protein was used as the immunogen for this CCND1 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CCND1 antibody is available for research use only.
• UniProt ID: P24385
• Storage: Store the CCND1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human mantle cell lymphoma stained with CCND1 antibody.

Western blot testing of 1) human HepG2, 2) mouse NIH3T3 and 3) mouse C2C12 lysate with CCND1 antibody. Expected/observed molecular weight ~32-36kDa.

Flow Cytometry testing of Jurkat cells. Black: cells alone; Green: isotype control; Red: PE-labeled Cyclin D1 antibody.

Flow Cytometry testing of MCF-7 cells. Black: cells alone; Green: isotype control; Red: PE-labeled Cyclin D1 antibody.