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    Cyclin B1 (phospho-S126) antibody

    Catalog Number: orb345436

    DispatchUsually dispatched within 5-10 working days
    $ 784.00
    Catalog Numberorb345436
    CategoryAntibodies
    DescriptionCyclin B1 (phospho-S126) antibody
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsELISA, IP, WB
    ReactivityHuman
    IsotypeIgG
    ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 115-140 of Human Cyclin B1 protein.
    Concentration2.01 mg/mL
    Dilution rangeELISA: 1:50,000, IP: 1:100, WB: 1:100 - 1:1,000
    Form/AppearanceLiquid (sterile filtered)
    PurityThis product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. This antibody is specific for phosphorylated human Cyclin B1 protein at the pS126 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Cyclin B1 from human. Cross reactivity may occur with Cyclin B1 from rat, dog and chimpanzee as only a single amino acid change was detected within the immunogen sequence from these sources (91% homology). Partial reactivity may also occur with Cyclin B1 from mouse and hamster where two amino acids changes was noted (83% homology). Minimal reactivity is expected with the non-phosphorylated form of the protein.
    ConjugationUnconjugated
    UniProt IDP14635
    StorageStore vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namesrabbit anti-Cyclin B1 pS126 Antibody, Cyclin B1, C
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    NoteFor research use only
    Application notesThis affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 48 kDa in size corresponding to Cyclin B1 by western blotting in the appropriate cell lysate or extract.
    Expiration Date12 months from date of receipt.
    Cyclin B1 (phospho-S126) antibody

    Western blot using Biorbyt's Affinity Purified anti-Cyclin B1 pS126 antibody shows detection of a band ~48 kDa corresponding to phosphorylated human Cyclin B1 (arrowheads) in various whole cell lysates. Lane 1: Hela (cervical carcinoma) [p/n orb348668], Lane 2: H23 (lung carcinoma), Lane 3: Hep3b (Hepatocarcinoma), Lane 4: T98G (Glioblastoma), and Lane 5: Daudi (B cell lymphoblast) [orb692723]. Each lane contains approximately 50 µg of lysates, separated by 12% SDS-PAGE using a 5% stack run at 100 volts until the dye front cleared the bottom of the gel. Transfer occurred overnight at 4°C at 15 mAmps. The membrane was blocked with 5% non-fat dry milk in TTBS (p/n orb348624) for 1 h at room temperature followed by addition of a 1:100 dilution of the antibody allowed to react for 2h at room temperature. After washes with TTBS a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG [H&L] MX (p/n orb347654) was added for 1 h at room temperature. After additional washes the membrane was incubated with ECL mix 1:1 for ~3 min. Excess detection solution was drained off and the membrane was exposed to Kodak film X-omat blue XB-1 for about 20 sec. Other detection systems will yield similar results.

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