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Catalog Number | orb345436 |
---|---|
Category | Antibodies |
Description | Cyclin B1 (phospho-S126) antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IP, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 115-140 of Human Cyclin B1 protein. |
Concentration | 2.01 mg/mL |
Dilution range | ELISA: 1:50,000, IP: 1:100, WB: 1:100 - 1:1,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. This antibody is specific for phosphorylated human Cyclin B1 protein at the pS126 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Cyclin B1 from human. Cross reactivity may occur with Cyclin B1 from rat, dog and chimpanzee as only a single amino acid change was detected within the immunogen sequence from these sources (91% homology). Partial reactivity may also occur with Cyclin B1 from mouse and hamster where two amino acids changes was noted (83% homology). Minimal reactivity is expected with the non-phosphorylated form of the protein. |
Conjugation | Unconjugated |
UniProt ID | P14635 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-Cyclin B1 pS126 Antibody, Cyclin B1, C Read more... |
Note | For research use only |
Application notes | This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 48 kDa in size corresponding to Cyclin B1 by western blotting in the appropriate cell lysate or extract. |
Expiration Date | 12 months from date of receipt. |
Western blot using Biorbyt's Affinity Purified anti-Cyclin B1 pS126 antibody shows detection of a band ~48 kDa corresponding to phosphorylated human Cyclin B1 (arrowheads) in various whole cell lysates. Lane 1: Hela (cervical carcinoma) [p/n orb348668], Lane 2: H23 (lung carcinoma), Lane 3: Hep3b (Hepatocarcinoma), Lane 4: T98G (Glioblastoma), and Lane 5: Daudi (B cell lymphoblast) [orb692723]. Each lane contains approximately 50 µg of lysates, separated by 12% SDS-PAGE using a 5% stack run at 100 volts until the dye front cleared the bottom of the gel. Transfer occurred overnight at 4°C at 15 mAmps. The membrane was blocked with 5% non-fat dry milk in TTBS (p/n orb348624) for 1 h at room temperature followed by addition of a 1:100 dilution of the antibody allowed to react for 2h at room temperature. After washes with TTBS a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG [H&L] MX (p/n orb347654) was added for 1 h at room temperature. After additional washes the membrane was incubated with ECL mix 1:1 for ~3 min. Excess detection solution was drained off and the membrane was exposed to Kodak film X-omat blue XB-1 for about 20 sec. Other detection systems will yield similar results.
IH, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IHC-P | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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