Cyclin B1 Antibody / CCNB1

• Catalog Number: orb749862
• Category: Antibodies
• Description: It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CCNB1/1098
• Tested applications: IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant human full-length protein was used as the immunogen for the Cyclin B1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Cyclin B1 antibody is available for research use only.
• UniProt ID: P14635
• Storage: Store the Cyclin B1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Cyclin B1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with Cyclin B1 antibody (CCNB1/1098).

IHC: Formalin-fixed, paraffin-embedded human testicular carcinoma stained with Cyclin B1 antibody.

IHC: Formalin-fixed, paraffin-embedded human tonsil stained with Cyclin B1 antibody.

IHC: Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Cyclin B1 antibody.

IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Cyclin B1 antibody.

Immunofluorescent staining of human MCF7 cells with Cyclin B1 antibody (clone CCNB1/1098, red), phalloidin (actin, green) and Hoechst (nuclei, blue).

SDS-PAGE analysis of purified, BSA-free Cyclin B1 antibody (clone CCNB1/1098) as confirmation of integrity and purity.