Cyclin B1 Antibody / CCNB1

• Catalog Number: orb248209
• Category: Antibodies
• Description: This antibody recognizes a protein of 55-62kDa, identified as Cyclin B1. In mammals, Cyclin B1 associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at amino acids Thr-14 and Tyr-15. This maintains the inactive state until the end of G2-phase. The inactive Cyclin B1-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates amino acids Thr-14 and Tyr-15 of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when Cyclin B1 is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. Cyclin B1-p34cdc2 plays a critical role in G2 to M transition.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: V92.1
• Tested applications: FACS, IF, IP
• Reactivity: Human, Mouse
• Isotype: Mouse IgG1, kappa
• Immunogen: Hamster protein was used as the immunogen for this Cyclin B1 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunoprecipitation: 1-2ug/500ug protein (precipitates active CDK1/cyclin B1 complexes)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Cyclin B1 antibody is available for research use only.
• Entrez: 891
• Storage: Store the Cyclin B1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed human HeLa cells with Cyclin B1 antibody (clone V92.1).

Flow cytometry testing of PFA-fixed human HeLa cells with Cyclin B1 antibody (clone V92.1); Red=isotype control, Blue=Cyclin B1 antibody.

SDS-PAGE analysis of purified, BSA-free Cyclin B1 antibody (clone V92.1) as confirmation of integrity and purity.