Cyclin Antibody Sampler Kit

• Catalog Number: orb342331
• Category: Assays and Kits
• Description: Cyclin Antibody Sampler Kit
• Tested applications: ELISA, IHC, IP, WB
• Concentration: n/a
• Dilution range: ELISA: 1:2,000 - 1:10,000, IP: 1:100, WB: 1:500 - 1:5,000
• Form/Appearance: Liquid (sterile filtered)
• Conjugation: Unconjugated
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Anti-Cyclin A Antibody, Anti-Cyclin B1 Antibody, A
• Note: For research use only
• Application notes: Each kit contains one unit of the following products: (100 µL) Anti-Cyclin A (RABBIT) Antibody (100 µL) Anti-Cyclin B1 (RABBIT) Antibody (100 µL) Anti-Cyclin D1 (RABBIT) Antibody (100 µL) Anti-Cyclin E (RABBIT) Antibody (100 µg) Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated
• Expiration Date: Please enquire.

Biorbyt's Anti-Cyclin A antibody was diluted 1:500 to detect Cyclin A in human testes tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal with a hematoxylin purple nuclear counter stain.

Biorbyt's anti-Cyclin B1 antibody was diluted 1:500 to detect Cyclin B1 in human brain cerebellum tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Western blot analysis is shown using Biorbyt's anti-Cyclin D1 antibody to detect Human Cyclin D1 present in asynchronous HN30 cell lysates. HN30 cells, are from head and neck cancer cells that over express cyclin B1 and D1. Comparison to a molecular weight marker indicates a band of ~34 kDa corresponding to the expected molecular weight for the protein (arrowhead). The blot was incubated with a 1:500 dilution of the antibody at room temperature. Detection occurred using a 1:10000 of HRP conjugated Goat-a-Rabbit IgG orb347654 and chemiluminescence reagent with a 1-min exposure time. Other detection systems will yield similar results.