CXCR3 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb704300
• Category: Antibodies
• Description: CXCR3 Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: 5F2
• Tested applications: ICC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant human CXCR3 protein
• Antibody Type: Recombinant Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, ICC/IF=1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 41 kDa
• Target: CXCR3
• UniProt ID: P49682
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CXCR3_HUMAN; Interferon-inducible protein 10 recep
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

A431 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (CXCR3) monoclonal Antibody, Unconjugated (orb704300) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (CXCR3) monoclonal Antibody, Unconjugated (orb704300) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

HUVEC cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (CXCR3) monoclonal Antibody, Unconjugated (orb704300) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Lane 1: Hela cell lysate, Primary: Anti-CXCR3 (orb704300) at 1:1000 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 41 kD, Observed band size: 43 kD.