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Catalog Number | orb485433 |
---|---|
Category | Antibodies |
Description | CX3CR1 antibody (PE) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC |
Predicted Reactivity | Bovine, Canine, Human, Rabbit |
Reactivity | Mouse, Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human CX3CR1 (151-250/355 aa) |
Concentration | 1mg/ml |
Dilution range | Flow-Cyt = 2ug/Test |
Form/Appearance | Liquid |
Purity | Affinity purified by Protein A |
Conjugation | PE |
Target | CX3CR1 |
UniProt ID | P49238 |
Storage | Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Buffer/Preservatives | 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol. |
Alternative names | Beta chemokine receptor-like 1; C X3 C CKR 1; CCRL Read more... |
Note | For research use only |
Application notes | Optimal dilutions/concentrations should be determined by the end user |
Expiration Date | 12 months from date of receipt. |
Blank control: U251. Primary Antibody (green line): Rabbit Anti-CX3CR1 antibody Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed
Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-CX3CR1 antibody Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed
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