CX3CR1 antibody

• Catalog Number: orb10490
• Category: Antibodies
• Description: CX3CR1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Canine, Human, Rabbit
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CX3CR1 (151-250/355 aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, Flow-Cyt=1ug/Test, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Purity: Affinity purified by Protein A
• Conjugation: Unconjugated
• MW: 39kDa
• Target: CX3CR1
• UniProt ID: P49238
• RRID: AB_10746442
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol
• Alternative names: Beta chemokine receptor-like 1; C X3 C CKR 1; CCRL
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Heart(Mouse) Lysate at 40 ug Heart(Rat) Lysate at 40 ug Primary: Anti-CX3CR1 at 1/1000 dilution Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50'55 kD Observed band size: 53 kD

Paraformaldehyde-fixed, paraffin embedded (rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CX3CR1) Polyclonal Antibody, Unconjugated at 1:1000 overnight at 4°C

Blank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-CX3CR1 antibody Dilution: 1ug/Test; Secondary Antibody: Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed