You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1272535 |
---|---|
Category | Antibodies |
Description | CX3CL1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFractalkine (human Fractalkine). |
Concentration | batch dependent |
Dilution range | ELISA:To detect hFractalkine by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hFractalkine. Sandwich: To detect hFractalkine by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human Fractalkine as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFractalkine.Western Blot:To detect hFractalkine by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFractalkine is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
Target | CX3CL1 |
UniProt ID | P78423 |
NCBI | P78423 |
Storage | Fractalkine antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
Alternative names | NTN, NTT, CXC3, CXC3C, SCYD1, ABCD-3, C3Xkine, fra Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
To detect hFractalkine by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFractalkine is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hFractalkine by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFractalkine is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas malignant ductal adenocarcinoma. The recommended concentration is 0.25ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
ELISA, FA, FACS, Kinetics | |
Human | |
Monoclonal | |
Unconjugated |
Filter by Rating