CUL9 antibody

• Catalog Number: orb750503
• Category: Antibodies
• Description: CUL9 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IP, WB
• Reactivity: Human
• Isotype: Antiserum
• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 2503-2517 of Human PARC (C-terminus) coupled to KLH.
• Concentration: 85 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: User Optimized, IP: User Optimized, WB: 1:500 - 1:1,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human and mouse PARC. Cross reactivity with PARC from other sources is not known.
• Conjugation: Unconjugated
• UniProt ID: Q8IWT3
• NCBI: Q8IWT3.2
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-PARC Antibody, p53 associated parkin l
• Note: For research use only
• Application notes: Anti-PARC reacts with human and mouse PARC by western blot and immunoprecipitation. The antibody immunoprecipitates protein from cell lysates (using HeLa, NIH-3T3, and others). To date co-immunoprecipitation using 35S-IP has been negative. A 281.2 kDa band corresponding to human PARC is detected. Most cell lines expressing PARC can be used as a positive control. Researchers should determine optimal titers for other applications.
• Expiration Date: 12 months from date of receipt.

Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin.