CUL4A antibody

• Catalog Number: orb750500
• Category: Antibodies
• Description: CUL4A antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IP, WB
• Reactivity: Human
• Isotype: Antiserum
• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal region near amino acids 1-25 of Human Cul4A coupled to KLH.
• Concentration: 85 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: User Optimized, IP: 1:500, WB: 1:500 - 1:1,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human Cullin 4A. Cross reactivity is expected against mouse Cul4A based on a high degree of sequence homology. Cross reactivity with other human cullins may occur.
• Conjugation: Unconjugated
• UniProt ID: Q13619
• NCBI: 38176422
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Cul4A Antibody, 2810470J21Rik antibody
• Note: For research use only
• Application notes: Anti-Cul4A has been tested by western blot, immunoprecipitation, and immunohistochemistry. The antibody immunoprecipitates protein from cell lysates (using HeLa, U2OS and others). This antibody also co-immunoprecipitates at least 8 subunits of COP9, DDB1 and CAND1. An 87.6 kDa band corresponding to human Cul4A is detected. Most cell lines expressing Cul4A can be used as a positive control. Researchers should determine optimal titers for other applications.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry. Biorbyt's anti-CUL4 antibody was diluted 1:500 to detect CUL4 in human testes tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin.

Western Blot of Rabbit Anti-Cul4 Antibody. Lane 1: Opal Pre-Stained Ladder. Lane 2: A431 whole cell lysate (p/n orb348665). Lane 3: U2OS whole cell lysate (p/n orb348676). Lane 4: HeLa whole cell lysate (p/n orb348668). Load: 35 µg lysate per lane. Primary Antibody: Anti-Cul4 at 1:500 overnight at 2-8°C. Secondary Antibody: Goat anti-rabbit IgG HRP (p/n orb347654) at 1:70000 at RT for 30 mins. Expect: ~87.6 kDa.

Western Blot of Rabbit Anti-Cul4 Antibody. Lane 1: Opal Pre-Stained Ladder. Lane 2: human Testis. Lane 3: human skeletal muscle. Load: 35 µg lysate per lane. Primary Antibody: Anti-Cul4 at 1:500 overnight at 2-8°C. Secondary Antibody: Goat anti-rabbit IgG HRP (p/n orb347654) at 1:70000 at RT for 30 mins. Expect: ~87.6 kDa.