CTNNB1 Antibody / Beta Catenin

• Catalog Number: orb1823680
• Category: Antibodies
• Description: Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CTNNB1/7759
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2, kappa
• Immunogen: A recombinant partial protein sequence (within amino acids 600-781) from the human protein was used as the immunogen for the Catenin Beta antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Catenin Beta antibody is available for research use only.
• UniProt ID: P35222
• Storage: Aliquot the Catenin Beta antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Analysis of a HuProt (TM) microarray containing more than 19, 000 full-length human proteins using Catenin Beta antibody (clone CTNNB1/7759). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

Western blot testing of human RH30 cell lysate with Catenin Beta antibody (clone CTNNB1/7759). Expected molecular weight: 85-95 kDa.

IHC staining of FFPE human salivary gland tissue with Catenin Beta antibody (clone CTNNB1/7759). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Catenin Beta antibody (clone CTNNB1/7759) as confirmation of integrity and purity.