CTLA4 Detection Set (Risk Free)

• Catalog Number: orb1227445
• Category: Assays and Kits
• Description: CTLA4 Detection Set (Risk Free)
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: ELISA, FC, ICC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Mouse monoclonal CTLA-4 antibodies were raised against the extracellular domain of human CTLA-4.
• Concentration: Antibody 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Storage: Antibodies can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: PBS containing 0.02% sodium azide.
• Note: For research use only
• Expiration Date: Please enquire.

Western blot analysis of CTLA-4 in overexpressing 293 cells using orb1239307, orb1239311, and orb1239312 antibody at 1, 0.5, and 0.25 μg/ml, respectively.

Immunocytochemistry of CTLA-4 in overexpressing 293 cells using (A) orb1239307, (B) orb1239311, (C) orb1239312, and (D) control mouse IgG antibody at 5 μg/ml.

Immunofluorescence of CTLA-4 in overexpressing 293 cells using (A) orb1239307, (B) orb1239311, (C) orb1239312, and (D) control mouse IgG antibody at 20 μg/ml.

Immunofluorescence of CTLA-4 in human tonsil tissue using (A) orb1239307, (B) orb1239311, (C) orb1239312, and (D) control mouse IgG antibody at at 20 μg/ml.

Immunohistochemistry of CTLA-4 in human lymph node tissue using (A) orb1239307, (B) orb1239311, (C) orb1239312 and (D) control mouse IgG antibody at 5 μg/ml.

Immunohistochemistry of CTLA-4 in human tonsil tissue using (A) orb1239307, (B) orb1239311, (C) orb1239312 and (D) control mouse IgG antibody at 5 μg/ml.

Titration curve analysis of CTLA-4 mAbs to detect recombinant CTLA-4 in ELISA with orb1239307, orb1239311, and orb1239312 antibodies at decreasing concentrations.

A sandwich ELISA was performed using the anti-CTLA-4 mAbs orb1239307, orb1239311, orb1239312, as the capture antibodies for the CTLA-4 extracellular domain antigen with HRP-labeled Risk-Free anti-CTLA-4 mAbs as the detection antibodies.