You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1239312 |
---|---|
Category | Antibodies |
Description | CTLA4 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 8A1 |
Tested applications | ELISA, ICC, IF, IHC-P, WB |
Reactivity | Human |
Isotype | IgG1 |
Immunogen | CTLA-4 antibody was raised against the extracellular domain of human CTLA-4. |
Concentration | 1 mg/mL |
Dilution range | CTLA-4 antibody can be used for detection of CTLA-4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Predicted: 25 kDa Observed: 32 kDa |
Target | CTLA4 |
UniProt ID | P16410 |
NCBI | NP_005205 |
Storage | CTLA-4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | CTLA-4 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol. |
Alternative names | CTLA-4 Antibody: CTL4, cytotoxic T-lymphocyte asso Read more... |
Note | For research use only |
Application notes | CTLA-4 antibody can be used for detection of CTLA-4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of CTLA-4 in overexpressing HEK293 cells CTLA-4 antibody at 0.5 and 1 μg/mL.
Immunocytochemistry of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 5 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 5 μg/mL.
Immunofluorescence of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 20 μg/mL. Red: CTLA4 Antibody [8A1] (orb1239312) Blue: DAPI staining
Immunofluorescence of CTLA-4 in human lymph node tissue with CTLA-4 antibody at 20 μg/mL.
Immunofluorescence of CTLA-4 in human tonsil tissue with CTLA-4 antibody at 20 μg/mL. Red: CTLA4 Antibody [8A1] (orb1239312) Blue: DAPI staining
Immunohistochemistry of CTLA-4 in human lymph node tissue with CTLA-4 antibody at 5 μg/mL.
Immunohistochemistry of CTLA-4 in human tonsil tissue with CTLA-4 antibody at 5 μg/mL.
Titration curve analysis of CTLA-4 mAbs to detect recombinant CTLA-4 in ELISA with orb1239307, orb1239311, and orb1239312 antibodies at decreasing concentrations.
ELISA, FACS, IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, Functional Studies, IF, IHC-P | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
Filter by Rating