You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1823831 |
---|---|
Category | Antibodies |
Description | Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) is a member of the CD28 superfamily and is a negative regulator of T cell-mediated immune responses. CTLA-4 expression is induced on the surface of T cells after CD28 binding and activation, and is constitutively expressed on T-regulatory cells, acting as an immune checkpoint inhibitor, downregulating T cell activity. CTLA-4 primarily inactivates T-cell activity by competing with the CD28 costimulatory molecule. CD28 and CTLA-4 share the identical ligands of CD80 and CD86 on antigenpresenting cells; and thus CTLA-4 competes with CD28 function in T-cell survival, proliferation, and recruitment. In particular, CTLA-4 down-modulates CD4+ helper T-cell activity and enhances Treg immunosuppressive functions. CTLA-4 has been shown to play a role in human diseases. CTLA-4 acts as a physiological brake on the activated immune system in order to maintain immune homeostasis. Several suppressive mechanisms for T-cell functions have been attributed to CTLA-4. |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | CTLA4/6867R |
Tested applications | IHC-P |
Reactivity | Human |
Isotype | Rabbit IgG, kappa |
Immunogen | A recombinant fragment of human protein was used as the immunogen for the Cytotoxic T-lymphocyte protein 4 antibody. |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
Conjugation | Unconjugated |
Formula | 1 mg/ml in 1X PBS; BSA free, sodium azide free |
Hazard Information | This Cytotoxic T-lymphocyte protein 4 antibody is available for research use only. |
UniProt ID | P16410 |
Storage | Aliquot the Cytotoxic T-lymphocyte protein 4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human tonsil tissue with Cytotoxic T-lymphocyte protein 4 antibody (clone CTLA4/6867R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with Cytotoxic T-lymphocyte protein 4 antibody (clone CTLA4/6867R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Cytotoxic T-lymphocyte protein 4 antibody (clone CTLA4/6867R) as confirmation of integrity and purity.
Filter by Rating