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Catalog Number | orb259602 |
---|---|
Category | Antibodies |
Description | CtBP1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, WB |
Predicted Reactivity | Hamster |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human CtBP1 (409-440aa HGLPPVAHPPHAPSPGQTVKPEADRDHASDQL), different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 47535 MW |
UniProt ID | Q13363 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | C-terminal-binding protein 1;CtBP1;1.1.1.-;CTBP1;C Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U20S cells using anti-CTBP1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of CTBP1 using anti-CTBP1 antibody.Lane 1:human SH-SY5Y cell;2:human MOLT-4 cell;3:human MCF-7 cell;4:rat PC-12 cell;5:mouse SP2/0 cell.
IF analysis of CTBP1 using anti-CTBP1 antibody.CTBP1 was detected in paraffin-embedded section of human intestinal cancer tissues.
IF analysis of CTBP1 using anti-CTBP1 antibody.CTBP1 was detected in paraffin-embedded section of rat intestine tissues.
IF analysis of CTBP1 using anti-CTBP1 antibody.CTBP1 was detected in immunocytochemical section of U20S cell.
IF analysis of CTBP1 using anti-CTBP1 antibody and anti-Tubulin Alpha antibody.CTBP1 was detected in immunocytochemical section of T47D cell.
IHC analysis of CTBP1 using anti-CTBP1 antibody.CTBP1 was detected in paraffin-embedded section of Mouse Thymus Tissue.
IHC analysis of CTBP1 using anti-CTBP1 antibody.CTBP1 was detected in paraffin-embedded section of Rat Spleen Tissue.
IHC analysis of CTBP1 using anti-CTBP1 antibody.CTBP1 was detected in paraffin-embedded section of Human Tonsil Tissue.
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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