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Catalog Number | orb1671811 |
---|---|
Category | Antibodies |
Description | CSF2RB Antibody |
Clonality | Recombinant |
Clone Number | BION-1 |
Tested applications | Blocking, Crystallography, FA, IHC, IP, WB |
Reactivity | Human |
Isotype | IgG kappa |
Immunogen | BALB/c mice were immunized intraperitonally with COS cells transfected DQP (Domain 4 of the common beta-chain) expression constructs. The immunizations were repeated 4 times at 2-week intervals. Four weeks after the final immunization, a mouse was boosted with COS transfectants intravenously. Three days later, splenocytes were harvested and fused with NS-1 myeloma cells. Hybridoma supernatants were screened on CHO DQP cells by flow cytometry, with untransfected CHO cells as a control. All antibodies were from single hybridoma clones as selected by a limiting dilution method. MoAbs were purified from ascites fluid or hybridoma supernatant by a protein A sepharose column. |
Concentration | batch dependent |
Conjugation | Unconjugated |
Target | CSF2RB |
UniProt ID | P32927 |
Storage | Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | CSF2RB, CD131, IL3RB, IL-3R common beta subunit, I Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow-cytometry using the Anti-CD131 antibody BION-1. U937 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control - black line) or the r version of BION-1 (orb1671811 - blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Immunofluorescence staining of U937 cells using anti-CD131 antibody (BION-1). Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of BION-1 (orb1671811) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671811- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.
ELISA, FA, FACS, Kinetics | |
Human | |
Monoclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, IHC, WB | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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