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Catalog Number | orb1272634 |
---|---|
Category | Antibodies |
Description | Csf2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Rat |
Immunogen | Produced from sera of rabbits immunized with highly pure recombinant Rat GM-CSF. Rat GM-CSF specific antibody was purified by affinity chromatography employing an immobilized Rat GM-CSF matrix. |
Concentration | batch dependent |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
Target | Csf2 |
UniProt ID | P48750 |
NCBI | P48750 |
Storage | The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
Alternative names | Gmcsf, Gm-csf, Csfgm, Granulocyte-macrophage colon Read more... |
Note | For research use only |
Application notes | ELISA:Sandwich:To detect Rat GM-CSF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with BIorbyt’s Biotinylated Anti-Rat GM-CSF (38-284) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat GM-CSF.Western Blot:To detect Rat GM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat GM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Expiration Date | 12 months from date of receipt. |
To detect Rat GM-CSF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat GM-CSF (orb1272623) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat GM-CSF.
To detect Rat GM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat GM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Rat GM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat GM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ICC, IF, IHC-P, WB | |
Guinea pig, Human, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P | |
Human | |
Rat | |
Monoclonal | |
Unconjugated |
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