CSF1 antibody

• Catalog Number: orb325141
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CSF1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-P, WB
• Predicted Reactivity: Canine, Guinea pig, Human, Mouse, Porcine, Rat
• Reactivity: Canine, Guinea pig, Human, Mouse, Porcine, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CSF1
• Concentration: Batch dependent within range: 100 μl at 0.5-1 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 61 kDa
• Target: CSF1
• UniProt ID: P09603
• Protein Sequence: Synthetic peptide located within the following region: SGSVLPLGELEGRRSTRDRRSPAEPEGGPASEGAARPLPRFNSVPLTDTG
• NCBI: NP_000748
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: anti MCSF antibody, anti MGC31930 antibody, anti C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of human heart tissue using CSF1 antibody

Western blot analysis of COLO205 cell lysate tissue using CSF1 antibody

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. Peptide is cross-reacting with CSF4 at ~59 kDa, and is also present in isoforms of CSF1 at ~56 kDa and ~51 kDa.

CSF1 in human placenta was detected using HRP/AEC red color stain, Working dilution 10 ug/mL.

Host: Rabbit, Target Name: CSF1, Sample Tissue: Human Fetal Liver, Antibody Dilution: 1.0 ug/mL.

Host: Rabbit, Target Name: CSF1, Sample Tissue: Human Jurkat Whole Cell, Antibody Dilution: 1 ug/mL.

Host: Rabbit, Target Name: CSF1, Sample Tissue: Human Liver Tumor, Antibody Dilution: 1 ug/mL.

Host: Rabbit, Target Name: CSF1, Sample Tissue: Human THP-1 Whole Cell, Antibody Dilution: 1 ug/mL.

Host: Rabbit, Target Name: WT1, Sample Type: 721_B, Antibody Dilution: 1.0 ug/mL, CSF1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.

Host: Rabbit, Target: CSF1, Positive control (+): Human liver (LI), Negative control (-): MCF7 (N10), Antibody concentration: 3 ug/mL.

WB Suggested Anti-CSF1 Antibody Titration: 0.2-1 ug/mL, ELISA Titer: 1:62500, Positive Control: COLO205 cell lysate.

WB Suggested Anti-CSF1 antibody Titration: 1 ug/mL, Sample Type: Human heart.