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Catalog Number | orb1257243 |
---|---|
Category | Antibodies |
Description | CSDE1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 369-600 of human CSDE1 (NP_009089.4). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IP: 1:20 - 1:50 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 102kDa |
Target | CSDE1 |
UniProt ID | O75534 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | Cold shock domain-containing protein E1, N-ras ups Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IP: 1:20 - 1:50 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using CSDE1 antibody (orb1257243) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunoprecipitation analysis of 100 ug extracts of HeLa cells using 3 ug CSDE1 antibody (orb1257243). Western blot was performed from the immunoprecipitate using CSDE1 antibody (orb1257243) at a dilution of 1:1000.
ICC, IHC-Fr, IHC-P, IP, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
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