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Catalog Number | orb1267138 |
---|---|
Category | Antibodies |
Description | CRTAP Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human |
Isotype | Rabbit Ig |
Immunogen | This CRTAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-59 amino acids from the N-terminal region of human CRTAP. |
Concentration | batch dependent |
Dilution range | For IF starting dilution is: 1:25For WB starting dilution is: 1:1000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 47 kDa |
Target | CRTAP |
UniProt ID | O75718 |
NCBI | O75718 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Cartilage-associated protein, CRTAP, CASP Read more... |
Note | For research use only |
Application notes | For IF starting dilution is: 1:25For WB starting dilution is: 1:1000 |
Expiration Date | 12 months from date of receipt. |
Fluorescent image of Hela cells stained with CRTAP Antibody (N-term). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Western blot analysis of lysates from HT-1080, ZR-75-1 cell line (from left to right), using CRTAP Antibody at 1:1000 at each lane.
Western blot analysis in Hela cell line lysates (35 ug/lane).
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